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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using ZBTB46 Polyclonal antibody (25455-1-AP)

    WB analysis of RAW 264.7 using 25455-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 25455-1-AP (ZBTB46 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using ZBTB46 Polyclonal antibody (25455-1-AP)

    IHC staining of mouse skin using 25455-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 25455-1-AP (ZBTB46 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using ZBTB46 Polyclonal antibody (25455-1-AP)

    IHC staining of mouse skin using 25455-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 25455-1-AP (ZBTB46 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ZBTB46 Polyclonal antibody

ZBTB46 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25455-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Zinc finger and BTB domain-containing protein 46, RINZF, BZEL, BTB-ZF protein expressed in effector lymphocytes, BTBD4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25455-1-AP targets ZBTB46 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB46 fusion protein Ag22273 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 46
Calculated molecular weight 589 aa, 64 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC073800
Gene Symbol ZBTB46
Gene ID (NCBI) 140685
RRIDAB_2880088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UZ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZBTB46, also named as BTBD4, ZNF340, is a 589 amino acid protein, which contains one BTB (POZ) domain and two C2H2-type zinc fingers. ZBTB46 functions as a transcriptional repressor for PRDM1.

Protocols

Product Specific Protocols
WB protocol for ZBTB46 antibody 25455-1-APDownload protocol
IHC protocol for ZBTB46 antibody 25455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols