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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZBTB32 Polyclonal antibody (12516-1-AP)

    WB analysis using 12516-1-AP (same clone as 12516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12516-1-AP (ZBTB32 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ZBTB32 Polyclonal antibody (12516-1-AP)

    IHC staining of mouse testis using 12516-1-AP (same clone as 12516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12516-1-AP (ZBTB32 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ZBTB32 Polyclonal antibody (12516-1-AP)

    IHC staining of mouse testis using 12516-1-AP (same clone as 12516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12516-1-AP (ZBTB32 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZBTB32 Polyclonal antibody (12516-1-AP)

    IF Staining of MCF-7 using 12516-1-AP (same clone as 12516-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB32 antibody (12516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 21738-1-AP 00069062 (red). This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12516-1-PBS targets ZBTB32 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB32 fusion protein Ag3199 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 32
Calculated molecular weight 487 aa, 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC017700
Gene Symbol ZBTB32
Gene ID (NCBI) 27033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Y4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The zinc finger and BTB domain containing 32 (ZBTB32) protein, also known as testis zinc finger protein (TZFP) or repressor of GATA (ROG), is a transcription factor belonging to the BTB/POZ-ZF protein family. It is encoded by the Zbtb32 gene, located on proximal chromosome 7 in the mouse genome. ZBTB32 was shown to ensure proper regulation of meiosis during spermatogenesis by acting as a repressor of the androgen receptor (PMID: 22851713, 34337361).