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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZBTB20 Polyclonal antibody (23987-1-AP)

    WB analysis using 23987-1-AP (same clone as 23987-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using ZBTB20 Polyclonal antibody (23987-1-AP)

    WB analysis of L02 using 23987-1-AP (same clone as 23987-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ZBTB20 Polyclonal antibody (23987-1-AP)

    WB analysis of HepG2 using 23987-1-AP (same clone as 23987-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ZBTB20 Polyclonal antibody (23987-1-AP)

    WB analysis of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ZBTB20 Polyclonal antibody (23987-1-AP)

    WB analysis of mouse brain using 23987-1-AP (same clone as 23987-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZBTB20 Polyclonal antibody (23987-1-AP)

    WB analysis of Jurkat using 23987-1-AP (same clone as 23987-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ZBTB20 Polyclonal antibody (23987-1-AP)

    WB analysis of HEK-293 using 23987-1-AP (same clone as 23987-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using ZBTB20 Polyclonal antibody (23987-1-AP)

    WB analysis of RAW 264.7 using 23987-1-AP (same clone as 23987-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZBTB20 Polyclonal antibody (23987-1-AP)

    IHC staining of human kidney using 23987-1-AP (same clone as 23987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZBTB20 Polyclonal antibody (23987-1-AP)

    IHC staining of human kidney using 23987-1-AP (same clone as 23987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ZBTB20 Polyclonal antibody (23987-1-AP)

    IF Staining of mouse brain using 23987-1-AP (same clone as 23987-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using ZBTB20 Polyclonal antibody (23987-1-AP)

    IF Staining of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using ZBTB20 Polyclonal antibody (23987-1-AP)

    IF Staining of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZBTB20 Polyclonal antibody, PBS Only

ZBTB20 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 23987-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Dendritic-derived BTB/POZ zinc finger protein, DPZF, Zinc finger and BTB domain-containing protein 20, Zinc finger protein 288, ZNF288

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23987-1-PBS targets ZBTB20 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21140

Product name: Recombinant human ZBTB20 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 313-668 aa of BC029041

Sequence: EPDSVEQQFGPGAARDSQAEPTQPEQAAEAPAEGGPQTNQLETGASSPERSNEVEMDSTVITVSNSSDKSVLQQPSVNTSIGQPLPSTQLYLRQTETLTSNLRMPLTLTSNTQVIGTAGNTYLPALFTTQPAGSGPKPFLFSLPQPLAGQQTQFVTVFQPGLSTFTAQLPAPQPLASSAGHSTASGQGEKKPYECTLCNKTFTAKQNYVKHMFVHTGEKPHQCSICWRSFSLKDYLIKHMVTHTGVRAYQCSICNKRFTQKSSLNVHMRLHRGEKSYECYICKKKFSHKTLLERHVALHSASNGTPPAGTPPGARAGPPGVVACTEGTTYVCSVCPAKFDQIEQFNDHMRMHVSDG

相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 20
Calculated molecular weight 668 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC029041
Gene Symbol ZBTB20
Gene ID (NCBI) 26137
RRIDAB_2879389
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9HC78
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZBTB20 (zinc finger and BTB domain containing 20), also known as HOF, DPZF, ODA-8S or ZNF288, is a 741 amino acid protein that localizes to the nucleus and contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed in thymus, spleen, lymph node and fetal liver, ZBTB20 exists as either a monomer or a homodimer that is thought to function as a transcription factor, playing a role in hematopoiesis, oncogenesis and immune responses. Multiple isoforms of ZBTB20 exist due to alternative splicing events.

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