Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293T cells, U-251 cells, U2OS cells, NIH/3T3 cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87621-1-RR targets ZBTB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag24041 Product name: Recombinant human ZBTB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 165-514 aa of BC020172 Sequence: RISQEQVPEASQLSQLTSNLAQVNRTNMTPSDPLQTSLSPELVSTPVPPPPPGEETNLEASSSDEQPASLTIAHVKPSIMKRNGSFPKYYACHLCGRRFTLRSSLREHLQIHTGVPFTSSQQGESRVPLTLCSNAADLGKDAMEVPEAGMISDSELQHISDSPIIDGQQQSETPPPSDIADIDNLEQADQEREVKRRKYECTICGRKFIQKSHWREHMYIHTGKPFKCSTCDKSFCRANQAARHVCLNQSIDTYTMVDKQTLELCTFEEGSQMDNMLVQTNKPYKCNLCDKTFSTPNEVVKHSCQNQNSDVFALDEGRSILLGSGDSEVTEPDHPVLASIKKEQETVLLD 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger and BTB domain containing 2 |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC020172 |
| Gene Symbol | ZBTB2 |
| Gene ID (NCBI) | 57621 |
| RRID | AB_3745656 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8N680 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZBTB2 (Zinc Finger and BTB Domain-Containing Protein 2) is a transcriptional regulator belonging to the POK (POZ/BTB and Krüppel) family of zinc finger proteins. It contains an N-terminal BTB (Broad-Complex, Tramtrack, and Bric-à-Brac) domain, which mediates protein-protein interactions and transcriptional repression, and a C-terminal set of Krüppel-type zinc fingers that recognize specific DNA sequences. ZBTB2 is involved in critical cellular processes, including cell cycle progression, differentiation, and DNA damage response.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZBTB2 antibody 87621-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |