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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ZBTB11 Polyclonal antibody (25215-1-AP)

    WB analysis of mouse liver using 25215-1-AP (same clone as 25215-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25215-1-AP (ZBTB11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using ZBTB11 Polyclonal antibody (25215-1-AP)

    WB analysis of PC-3 using 25215-1-AP (same clone as 25215-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25215-1-AP (ZBTB11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using ZBTB11 Polyclonal antibody (25215-1-AP)

    IHC staining of human urothelial carcinoma using 25215-1-AP (same clone as 25215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25215-1-AP (ZBTB11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using ZBTB11 Polyclonal antibody (25215-1-AP)

    IHC staining of human urothelial carcinoma using 25215-1-AP (same clone as 25215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25215-1-AP (ZBTB11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25215-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZBTB11 Polyclonal antibody, PBS Only

ZBTB11 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25215-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Zinc finger and BTB domain-containing protein 11, ZNF U69274

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25215-1-PBS targets ZBTB11 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17000

Product name: Recombinant human ZBTB11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 704-1053 aa of BC111700

Sequence: QKQFQCELCVKSFVTKRSLQEHMSIHTGESKYLCSVCGKSFHRGSGLSKHFKKHQPKPEVRGYHCTQCEKSFFEARDLRQHMNKHLGVKPFQCQFCDKCYSWKKDWYSHVKSHSVTEPYRCNICGKEFYEKALFRRHVKKATHGKKGRAKQNLERVCEKCGRKFTQLREYRRHMNNHEGVKPFECLTCGVAWADARSLKRHVRTHTGERPYVCPVCSEAYIDARTLRKHMTKFHRDYVPCKIMLEKDTLQFHNQGTQVAHAVSILTAGMQEQESSGPQELETVVVTGETMEALEAVAATEEYPSVSTLSDQSIMQVVNYVLAQQQGQKLSEVAEAIQTVKVEVAHISGGE

相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 11
Calculated molecular weight 1053 aa, 119 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC111700
Gene Symbol ZBTB11
Gene ID (NCBI) 27107
RRIDAB_2879964
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95625
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Zinc finger and BTB domain containing 11 (ZBTB11), also named as ZNF U69274, is a 1053 amino acid protein, which contains one BTB domain and twelve C2H2-type zinc fingers. ZBTB11 localizes in the nucleus and may be involved in transcriptional regulation. The molecular weight of ZBTB11 is 119 kDa, but the phosphorylated ZBTB11 is a 135-140 kDa protein.

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