Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, rat brain tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23958-1-AP targets ZBBX in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20988 Product name: Recombinant human ZBBX protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-326 aa of BC034229 Sequence: MEKVQLEFENQEMEKKLQEFRSTRNKEKEDRESSEYYWKSGKVGKLVNQSYMMSQNKGNVVKFSAGKVKLKLLKEQIQEPVKPTVNYKMANSSECEKPKINGKVCGQCENKAALLVCLECGEDYCSGCFANVHQKGALKLHRTTLLQARSQILFNVLDVAHQFIKDVNPDEPKEENNSTKETSKIQHKPKSVLLQRSSSEVEITTMKRAQRTKPRKSLLCEGSFDEEASAQSFQEVLSQWRTGNHDDNKKQNLHAAVKDSLEECEVQTNLKIWREPLNIELKEDILSYMEKLWLKKHRRTPQEQLFKCYQIRSHIHMKPLVMHSVL 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, B-box domain containing |
| Calculated molecular weight | 800 aa, 91 kDa |
| Observed molecular weight | 88-96 kDa |
| GenBank accession number | BC034229 |
| Gene Symbol | ZBBX |
| Gene ID (NCBI) | 79740 |
| RRID | AB_3742099 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | A8MT70 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZBBX (Zinc finger B-box domain-containing protein 1) contains a classic B-box-type zinc-finger (B-box1) motif that adopts a C6H2 zinc-binding fold and is therefore predicted to bind zinc ions and mediate protein-protein or protein-nucleic acid interactions. ZBBX was implicated previously by a de novo deletion in a male ADHD proband, and separately by a CNV gain disrupting ZBBX (PMID: 21832240).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZBBX antibody 23958-1-AP | Download protocol |
| WB protocol for ZBBX antibody 23958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |