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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis of Jurkat using 15592-1-AP

    WB result of ZAP70 antibody (15592-1-AP, 1:20000) with si-control and si-ZAP70 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis using 15592-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis of A375 using 15592-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis of Jurkat using 15592-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis of A375 using 15592-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using ZAP70 Polyclonal antibody (15592-1-AP)

    IP experiment of Jurkat using 15592-1-AP

    IP result of anti-ZAP70 (IP:15592-1-AP, 4ug; Detection:15592-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human tonsillitis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human tonsillitis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human lymphoma using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human lymphoma using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human kidney using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human kidney using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human testis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human testis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using ZAP70 Polyclonal antibody (15592-1-AP)

    IF Staining of RAW 264.7 using 15592-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using ZAP70 antibody (15592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15592-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK, STD

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, A375 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human lymphoma tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15592-1-AP targets ZAP70 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7943

Product name: Recombinant human ZAP70 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 149-493 aa of BC039039

Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA

相同性解析による交差性が予測される生物種
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_2218654
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43403
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

Product Specific Protocols
IF protocol for ZAP70 antibody 15592-1-APDownload protocol
IHC protocol for ZAP70 antibody 15592-1-APDownload protocol
IP protocol for ZAP70 antibody 15592-1-APDownload protocol
WB protocol for ZAP70 antibody 15592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol Methods

An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination

Authors - Jiaqi Zheng
View Article
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