Validation Data Gallery
Tested Applications
| Positive WB detected in | human kidney tissue, human placenta tissue, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66839-1-Ig targets YY2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25373 Product name: Recombinant human YY2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-163 aa of BC137217 Sequence: MASNEDFSITQDLEIPADIVELHDINVEPLPMEDIPTESVQYEDVDGNWIYGGHNHPPLMVLQPLFTNTGYGDHDQEMLMLQTQEEVVGYCDSDNQLGNDLEDQLALPDSIEDEHFQMTLASLSASAASTSTSTQSRSKKPSKKPSGKSATSTEANPAGSSSS 相同性解析による交差性が予測される生物種 |
| Full Name | YY2 transcription factor |
| Calculated molecular weight | 372 aa, 46 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC137217 |
| Gene Symbol | YY2 |
| Gene ID (NCBI) | 404281 |
| RRID | AB_2882182 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15391 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YY2 (Yin-Yang 2) encodes a zinc finger transcription factor similarity to the well-characterized YY1 that locating to nucleus. Both proteins recognize an identical DNA-binding motifs with a high homologous Zinc Finger region, and these transcription factors can act as activators or repressors when bound to DNA (PMID:26350623). It is reported that YY2 is placental mammal-specific and is absent in marsupial and non-mammalian vertebrate species (PMID:16377127). YYs are play an important role in multiple developmental processes , such as implantation, cell differentiation, X inactivation, imprinting and organogenesis (PMID:27191592). YY2 is expressed in kindey, liver, spleen and testicle but not in the colon (PMID:15087442). A 41kDa band could be detected.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YY2 antibody 66839-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |