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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YWHAZ Monoclonal antibody (67698-1-Ig)

    WB analysis using 67698-1-Ig (same clone as 67698-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67698-1-Ig (YWHAZ antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using YWHAZ Monoclonal antibody (67698-1-Ig)

    IHC staining of human lung cancer using 67698-1-Ig (same clone as 67698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67698-1-Ig (YWHAZ antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using YWHAZ Monoclonal antibody (67698-1-Ig)

    IHC staining of human liver cancer using 67698-1-Ig (same clone as 67698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67698-1-Ig (YWHAZ antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using YWHAZ Monoclonal antibody (67698-1-Ig)

    IF Staining of MCF-7 using 67698-1-Ig (same clone as 67698-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using YWHAZ antibody (67698-1-Ig, Clone: 3G12B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using YWHAZ Monoclonal antibody (67698-1-Ig)

    FC experiment of A549 using 67698-1-Ig (same clone as 67698-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.2 μg YWHAZ Monoclonal antibody (67698-1-Ig, Clone:3G12B10) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005), and 0.2 μg Mouse IgM Isotype Control (65243-1-Ig, Clone: MOPC-104E) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

YWHAZ Monoclonal antibody, PBS Only

YWHAZ Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67698-1-PBS
Clone No.3G12B10

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

14-3-3, 14-3-3 protein zeta/delta, 3G12B10, KCIP 1, KCIP-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67698-1-PBS targets YWHAZ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6667

Product name: Recombinant human YWHAZ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-245 aa of BC003623

Sequence: MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN

相同性解析による交差性が予測される生物種
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Calculated molecular weight 28 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC003623
Gene Symbol YWHAZ
Gene ID (NCBI) 7534
RRIDAB_2882890
Conjugate Unconjugated
Form
FormLiquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP63104
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

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