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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YWHAZ Polyclonal antibody (14881-1-AP)

    WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using YWHAZ Polyclonal antibody (14881-1-AP)

    WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using YWHAZ Polyclonal antibody (14881-1-AP)

    WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using YWHAZ Polyclonal antibody (14881-1-AP)

    WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using YWHAZ Polyclonal antibody (14881-1-AP)

    IHC staining of mouse brain using 14881-1-AP (same clone as 14881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14881-1-AP (YWHAZ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using YWHAZ Polyclonal antibody (14881-1-AP)

    IHC staining of human skin cancer using 14881-1-AP (same clone as 14881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14881-1-AP (YWHAZ antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

YWHAZ Polyclonal antibody, PBS Only

YWHAZ Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14881-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

14-3-3, 14-3-3 protein zeta/delta, KCIP 1, KCIP-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14881-1-PBS targets YWHAZ in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6654

Product name: Recombinant human YWHAZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-245 aa of BC003623

Sequence: MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN

相同性解析による交差性が予測される生物種
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC003623
Gene Symbol YWHAZ
Gene ID (NCBI) 7534
RRIDAB_2218248
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP63104
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAZ.

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