Validation Data Gallery
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Tested Applications
| Positive WB detected in | HCT 116 cells, PC-12 cells, C6 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84709-3-RR targets YTHDF3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag22407 Product name: Recombinant human YTHDF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 284-351 aa of BC052970 Sequence: SVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQ 相同性解析による交差性が予測される生物種 |
| Full Name | YTH domain family, member 3 |
| Calculated molecular weight | 585 aa, 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC052970 |
| Gene Symbol | YTHDF3 |
| Gene ID (NCBI) | 253943 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7Z739 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDF3 antibody 84709-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
