Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 27779-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27779-1-AP (YTHDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 27779-1-AP
WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HepG2 cells.
WB analysis of HeLa using 27779-1-AP
WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HeLa cells.
IP experiment of HeLa using 27779-1-AP
IP result of anti-YTHDC2 (IP:27779-1-AP, 4ug; Detection:27779-1-AP 1:5000) with HeLa cells lysate 1200 ug.
IHC staining of rat testis using 27779-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 27779-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 27779-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 27779-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using YTHDC2 antibody (27779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HeLa using 27779-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human YTHDC2 (27779-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

YTHDC2 Polyclonal antibody

YTHDC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 27779-1-AP

Publications(36)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

3'-5' RNA helicase YTHDC2, EC:3.6.4.13, hYTHDC2, YTH domain-containing protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (36)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	rat testis tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 29 publications below
IHC	See 1 publications below
IF	See 8 publications below
IP	See 1 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

27779-1-AP targets YTHDC2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26690 Product name: Recombinant human YTHDC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1131-1430 aa of BC137285 Sequence: HSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSSADTEFSDECTTAERVLMKSPSPALHPPQKYKDRGILHPKRGTEDRSDQSSLKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPVRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSIVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPLVGEQLLQLWERLPLGEKNTTD 相同性解析による交差性が予測される生物種
Full Name	YTH domain containing 2
Observed molecular weight	160 kDa
GenBank accession number	BC137285
Gene Symbol	YTHDC2
Gene ID (NCBI)	64848
RRID	AB_2880970
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H6S0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

Protocols

Product Specific Protocols
IF protocol for YTHDC2 antibody 27779-1-AP	Download protocol
IHC protocol for YTHDC2 antibody 27779-1-AP	Download protocol
IP protocol for YTHDC2 antibody 27779-1-AP	Download protocol
WB protocol for YTHDC2 antibody 27779-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol Inhibition of METTL3 in macrophages provides protection against intestinal inflammation Authors - Huilong Yin View Article
human	IF	Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. Authors - Hagai Marmor-Kollet View Article
human	WB	Sci Adv Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A). Authors - Ji Hoon Lee View Article
human	WB	Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis. Authors - Shujie Chen View Article
human	WB,RIP	Cancer Lett LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation Authors - Lu Chen KD Validated View Article
mouse	WB,IP	Genes Dev YTHDC2 control of gametogenesis requires helicase activity but not m6A binding. Authors - Yuhki Saito View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27779-1-AP

WB analysis of HepG2 using 27779-1-AP

WB analysis of HeLa using 27779-1-AP

IP experiment of HeLa using 27779-1-AP

IHC staining of rat testis using 27779-1-AP

IHC staining of rat testis using 27779-1-AP

IHC staining of human stomach cancer using 27779-1-AP

IF Staining of HepG2 using 27779-1-AP

FC experiment of HeLa using 27779-1-AP

YTHDC2 Polyclonal antibody

YTHDC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inhibition of METTL3 in macrophages provides protection against intestinal inflammation

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation

YTHDC2 control of gametogenesis requires helicase activity but not m6A binding.