Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse tissue |
| Positive IHC detected in | mouse brain tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 41 publications below |
| IHC | See 1 publications below |
| IF | See 8 publications below |
| IP | See 4 publications below |
| CoIP | See 1 publications below |
| RIP | See 12 publications below |
Product Information
14392-1-AP targets YTHDC1 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5882 Product name: Recombinant human YTHDC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 360-709 aa of BC053863 Sequence: STLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLFPPDESIDLYQVIHKMRHKRRMHSQPRSRGRPSRREPVRDVGRRRPEDYDIHNSRKKPRIDYPPEFHQRPGYLKDPRYQEVDRRFSGVRRDVFLNGSYNDYVREFHNMGPPPPWQGMPPYPGMEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERLCDRDRDRGERGRYRR 相同性解析による交差性が予測される生物種 |
| Full Name | YTH domain containing 1 |
| Calculated molecular weight | 85 kDa |
| Observed molecular weight | ~100 kDa |
| GenBank accession number | BC053863 |
| Gene Symbol | YTHDC1 |
| Gene ID (NCBI) | 91746 |
| RRID | AB_2878052 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YTHDC1, also named as KIAA1966 or YT521, is a 727 animo acid protein, which contains 1 YTH domain. YTHDC1 localizes in the nucleus and Interacts with EMD, KHDRBS1/SAM68, KHDRBS3, TRA2B/SFRS10, SC35 and SF2. YTHDC1 as a RNA-binding protein that regulates alternative splice site selection. The calcualted molecular weight of YTHDC1 is 85 kDa, but modified TFEB is about 110 kDa (PMID: 10973987 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for YTHDC1 antibody 14392-1-AP | Download protocol |
| WB protocol for YTHDC1 antibody 14392-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis. | ||
Nucleic Acids Res N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication. | ||
Cell Death Differ YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect. | ||
Dev Cell N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation. | ||
J Neuroinflammation m6A-methylase METTL3 promotes retinal angiogenesis through modulation of metabolic reprogramming in RPE cells |
