Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse bone marrow, mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33153-1-AP targets YM-1+YM-2 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag39745 Product name: Recombinant mouse CHIL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-368 aa of NM_009892 Sequence: YQLMCYYTSWAKDRPIEGSFKPGNIDPCLCTHLIYAFAGMQNNEITYTHEQDLRDYEALNGLKDKNTELKTLLAIGGWKFGPAPFSAMVSTPQNRQIFIQSVIRFLRQYNFDGLNLDWQYPGSRGSPPKDKHLFSVLVKEMRKAFEEESVEKDIPRLLLTSTGAGIIDVIKSGYKIPELSQSLDYIQVMTYDLHDPKDGYTGENSPLYKSPYDIGKSADLNVDSIISYWKDHGAASEKLIVGFPAYGHTFILSDPSKTGIGAPTISTGPPGKYTDESGLLAYYEVCTFLNEGATEVWDAPQEVPYAYQGNEWVGYDNVRSFKLKAQWLKDNNLGGAVVWPLDMDDFSGSFCHQRHFPLTSTLKGDLNIHSASCKGPY 相同性解析による交差性が予測される生物種 |
| Full Name | chitinase 3-like 3 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | NM_009892 |
| Gene Symbol | Chi3l3 |
| Gene ID (NCBI) | 12655 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O35744 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Chitinase-like protein 3 (Chil3), also called ECF-L or Ym1, is a member of chitinase-like protein family which has chemotactic activity for eosinophils (PMID: 27256220). CHIL3 promotes NSC self-renewal and commitment to the neuronal lineage. Endogenous expression of CHIL3 is observed in the neurogenic niche of the mouse brain and retina and in lesions of those injury models (PMID: 36368330). Chitinase-like protein 4 (Chil4), also called Ym2, regulates regeneration of the injured OE in vivo in both male and female mice and cell proliferation/differentiation in OE colonies in vitro (PMID: 34016714). This antibody detects both YM1 and YM2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YM-1+YM-2 antibody 33153-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |