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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using YLPM1 Polyclonal antibody (21332-1-AP)

    WB analysis of SH-SY5Y using 21332-1-AP (same clone as 21332-1-PBS)

    WB result of YLPM1 antibody (21332-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YLPM1 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 21332-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using YLPM1 Polyclonal antibody (21332-1-AP)

    WB analysis using 21332-1-AP (same clone as 21332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21332-1-AP (YLPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21332-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Neuro-2a cells using YLPM1 Polyclonal antibody (21332-1-AP)

    IP experiment of Neuro-2a using 21332-1-AP (same clone as 21332-1-PBS)

    IP result of anti-YLPM1 (IP:21332-1-AP, 4ug; Detection:21332-1-AP 1:1000) with Neuro-2a cells lysate 1440 ug. This data was developed using the same antibody clone with 21332-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using YLPM1 Polyclonal antibody (21332-1-AP)

    IF Staining of SH-SY5Y using 21332-1-AP (same clone as 21332-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using YLPM1 antibody (21332-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21332-1-PBS in a different storage buffer formulation.

YLPM1 Polyclonal antibody, PBS Only

YLPM1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 21332-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

C14orf170, YLP motif-containing protein 1, YLPM 1, ZAP, ZAP113

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21332-1-PBS targets YLPM1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15408

Product name: Recombinant human YLPM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1960-2146 aa of BC023570

Sequence: MSADNQTCGKRNIHGRKLKEINKMADHWETAPRHMMRLDIRSLLQDAAIEEVEMEDFDANIEEQKEEKKDAEEEESELGYIPKSKWEMDTSEAKLDKLDGLRTGTKRKRDWEAIASRMEDYLQLPDDYDTRASEPGKKRVRWADLEEKKDADRKRAIGFVVGQTDWEKITDESGHLAEKALNRTKYI

相同性解析による交差性が予測される生物種
Full Name YLP motif containing 1
Calculated molecular weight 1951 aa, 220 kDa
Observed molecular weight200 kDa, 250-290 kDa
GenBank accession numberBC023570
Gene Symbol YLPM1
Gene ID (NCBI) 56252
RRIDAB_3742028
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49750
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

YLPM1 also known as ZAP3, ZAP113, or C14orf170. YLPM1 plays a critical role in the regulation of telomerase activity and transcriptional repression. The molecular weight of the human YLPM1 protein is approximately 250-300 kDa, with a shorter isoform about 200 kDa.

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