Validation Data Gallery
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Tested Applications
| Positive WB detected in | SK-N-SH cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16406-1-AP targets YIF1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | YIF1B fusion protein Ag9538 相同性解析による交差性が予測される生物種 |
| Full Name | Yip1 interacting factor homolog B (S. cerevisiae) |
| Calculated molecular weight | 294 aa, 32 kDa |
| Observed molecular weight | 31~34 kDa |
| GenBank accession number | BC014974 |
| Gene Symbol | YIF1B |
| Gene ID (NCBI) | 90522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5BJH7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YIF1B is an intracellular membrane-bound protein belonging to the Yip family, a trafficking protein orthologue of YIF1P that was initially identified in Saccharomyces cerevisiae as interacting with YIP1P and essential for secretion. YIF1B is expressed in various tissues, especially in brain neurons (PMID: 33103737). YIF1B is localized in the intermediate compartment between the endoplasmic reticulum (ER) and the Golgi apparatus (PMID: 26077767). Western blot analysis detected YIF1B at an apparent molecular mass of 31 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YIF1B antibody 16406-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |