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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YES1-Specific Polyclonal antibody (20243-1-AP)

    WB analysis using 20243-1-AP (same clone as 20243-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20243-1-AP (YES1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using YES1-Specific Polyclonal antibody (20243-1-AP)

    WB analysis of HepG2 using 20243-1-AP (same clone as 20243-1-PBS)

    WB result of YES1-Specific antibody (20243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1-Specific transfected HepG2 cells. This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using YES1-Specific Polyclonal antibody (20243-1-AP)

    IP experiment of A549 using 20243-1-AP (same clone as 20243-1-PBS)

    IP result of anti-YES1-Specific (IP:20243-1-AP, 4ug; Detection:20243-1-AP 1:500) with A549 cells lysate 1400 ug. This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using YES1-Specific Polyclonal antibody (20243-1-AP)

    IHC staining of human lung cancer using 20243-1-AP (same clone as 20243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using YES1-Specific Polyclonal antibody (20243-1-AP)

    IHC staining of human lung cancer using 20243-1-AP (same clone as 20243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YES1-Specific Polyclonal antibody (20243-1-AP)

    IHC staining of human colon cancer using 20243-1-AP (same clone as 20243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YES1-Specific Polyclonal antibody (20243-1-AP)

    IHC staining of human colon cancer using 20243-1-AP (same clone as 20243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20243-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

YES1-Specific Polyclonal antibody, PBS Only

YES1-Specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20243-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

YES1, c yes, EC:2.7.10.2, HsT441, P61 YES

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20243-1-PBS targets YES1-Specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberNM_005433
Gene Symbol YES1
Gene ID (NCBI) 7525
RRIDAB_10697656
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07947
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody is specific to YES1.

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