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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YES1 Monoclonal antibody (67196-1-Ig)

    WB analysis using 67196-1-Ig (same clone as 67196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67196-1-Ig (YES1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using YES1 Monoclonal antibody (67196-1-Ig)

    WB analysis of HepG2 using 67196-1-Ig (same clone as 67196-1-PBS)

    WB result of YES1 antibody (67196-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1 transfected HepG2 cells. This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using YES1 Monoclonal antibody (67196-1-Ig)

    IHC staining of human kidney using 67196-1-Ig (same clone as 67196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using YES1 Monoclonal antibody (67196-1-Ig)

    IHC staining of human kidney using 67196-1-Ig (same clone as 67196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

YES1 Monoclonal antibody, PBS Only

YES1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67196-1-PBS
Clone No.1A12E7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67196-1-PBS targets YES1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5016

Product name: Recombinant human YES1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 275-526 aa of BC048960

Sequence: ESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSFL

相同性解析による交差性が予測される生物種
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Calculated molecular weight 61 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC048960
Gene Symbol YES1
Gene ID (NCBI) 7525
RRIDAB_2918483
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP07947
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.