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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using YAF2 Polyclonal antibody (13980-1-AP)

    WB analysis of HEK-293 using 13980-1-AP (same clone as 13980-1-PBS)

    WB result of YAF2 antibody (13980-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-YAF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using YAF2 Polyclonal antibody (13980-1-AP)

    IP experiment of Jurkat using 13980-1-AP (same clone as 13980-1-PBS)

    IP result of anti-YAF2 (IP:13980-1-AP, 4ug; Detection:13980-1-AP 1:800) with Jurkat cells lysate 1920 ug. This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using YAF2 Polyclonal antibody (13980-1-AP)

    IP experiment of HeLa using 13980-1-AP (same clone as 13980-1-PBS)

    IP result of anti-YAF2 (IP:13980-1-AP, 4ug; Detection:13980-1-AP 1:600) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using YAF2 Polyclonal antibody (13980-1-AP)

    IHC staining of mouse brain using 13980-1-AP (same clone as 13980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13980-1-AP (YAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using YAF2 Polyclonal antibody (13980-1-AP)

    IHC staining of mouse brain using 13980-1-AP (same clone as 13980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13980-1-AP (YAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using YAF2 Polyclonal antibody (13980-1-AP)

    IF Staining of U2OS using 13980-1-AP (same clone as 13980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YAF2 antibody (13980-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YAF2 Polyclonal antibody (13980-1-AP)

    IF Staining of HeLa using 13980-1-AP (same clone as 13980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using YAF2 antibody (13980-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

YAF2 Polyclonal antibody, PBS Only

YAF2 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13980-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, Indirect ELISA

Synonyms

YY1 associated factor 2, YY1-associated factor 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13980-1-PBS targets YAF2 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5023

Product name: Recombinant human YAF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC037777

Sequence: MGDKKSPTRPKRQPKPSSDEGYWDCSVCTFRNSAEAFKCMMCDVRKGTSTRSTLFEVIVSASRTKEPLKFPISGRKPRPVSQLVAQQVTQQFVPPTQSKKEKKDKVEKEKSEKETTSKKNSHKKTRPRLKNVDRSSAQHLEVTVGDLTVIITDFKEKTKSPPASSAASADQHSQSGSSSDNTERGMSRSSSPRGEASSLNGESH

相同性解析による交差性が予測される生物種
Full Name YY1 associated factor 2
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight20-30 kDa
GenBank accession numberBC037777
Gene Symbol YAF2
Gene ID (NCBI) 10138
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY57
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).

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