Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26541-1-AP targets XYLB in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19208 Product name: Recombinant human XYLB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 185-536 aa of BC137076 Sequence: EAYSHTERISLVSSFAASLFLGSYSPIDYSDGSGMNLLQIQDKVWSQACLGACAPHLEEKLSPPVPSCSVVGAISSYYVQRYGFPPGCKVVAFTGDNPASLAGMRLEEGDIAVSLGTSDTLFLWLQEPMPALEGHIFCNPVDSQHYMALLCFKNGSLMREKIRNESVSRSWSDFSKALQSTEMGNGGNLGFYFDVMEITPEIIGRHRFNTENHKVAAFPGDVEVRALIEGQFMAKRIHAEGLGYRVMSKTKILATGGASHNREILQVLADVFDAPVYVIDTANSACVGSAYRAFHGLAGGTDVPFSEVVKLAPNPRLAATPSPGASQVYEALLPQYAKLEQRILSQTRGPPE 相同性解析による交差性が予測される生物種 |
| Full Name | xylulokinase homolog (H. influenzae) |
| Calculated molecular weight | 536 aa, 58 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC137076 |
| Gene Symbol | XYLB |
| Gene ID (NCBI) | 9942 |
| RRID | AB_2880546 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75191 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human xylulokinase-like protein (XYLB), is a 58 kDa protein belongs to a family of enzymes that include fucokinase, gluconokinase, glycerokinase and xylulokinase. XYLB is a key regulator for controlling metabolic disease.XYLB also plays important roles in energy metabolism. XYLB has 2 isoforms produced by alternative splicing with the MW of 58 and 43 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for XYLB antibody 26541-1-AP | Download protocol |
| WB protocol for XYLB antibody 26541-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
