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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XRCC3 Monoclonal antibody (67222-1-Ig)

    WB analysis using 67222-1-Ig (same clone as 67222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using XRCC3 Monoclonal antibody (67222-1-Ig)

    WB analysis of HT-29 using 67222-1-Ig (same clone as 67222-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using XRCC3 Monoclonal antibody (67222-1-Ig)

    WB analysis of Caco-2 using 67222-1-Ig (same clone as 67222-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using XRCC3 Monoclonal antibody (67222-1-Ig)

    WB analysis of HT-1080 using 67222-1-Ig (same clone as 67222-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using XRCC3 Monoclonal antibody (67222-1-Ig)

    IP experiment of MCF-7 using 67222-1-Ig (same clone as 67222-1-PBS)

    IP result of anti-XRCC3 (IP:67222-1-Ig, 5ug; Detection:67222-1-Ig 1:500) with MCF-7 cells lysate 640 ug. This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IHC staining of mouse testis using 67222-1-Ig (same clone as 67222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IHC staining of human placenta using 67222-1-Ig (same clone as 67222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IHC staining of human placenta using 67222-1-Ig (same clone as 67222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IHC staining of mouse testis using 67222-1-Ig (same clone as 67222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IF Staining of mouse testis using 67222-1-Ig (same clone as 67222-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using XRCC3 antibody (67222-1-Ig, Clone: 3A12F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IF Staining of mouse testis using 67222-1-Ig (same clone as 67222-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using XRCC3 antibody (67222-1-Ig, Clone: 3A12F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67222-1-PBS in a different storage buffer formulation.

XRCC3 Monoclonal antibody, PBS Only

XRCC3 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 67222-1-PBS
Clone No.3A12F9

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

DNA repair protein XRCC3, XRCC3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67222-1-PBS targets XRCC3 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag13317

Product name: Recombinant human XRCC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC011725

Sequence: MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQQKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYICTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLVVIDSVAAPFRCEFDSQASAPRARHLQSLGAMLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERVSPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH

相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 3
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC011725
Gene Symbol XRCC3
Gene ID (NCBI) 7517
RRIDAB_2882513
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43542
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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