Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, K-562 cells, U2OS cells |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87251-1-RR targets XRCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15172 Product name: Recombinant human XRCC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 284-633 aa of BC023593 Sequence: TAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSQRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA 相同性解析による交差性が予測される生物種 |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 1 |
| Calculated molecular weight | 633 aa, 70 kDa |
| Observed molecular weight | 80-90 kDa |
| GenBank accession number | BC023593 |
| Gene Symbol | XRCC1 |
| Gene ID (NCBI) | 7515 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18887 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
XRCC1 (X-ray Repair Cross Complementing group 1) is a molecular scaffold protein that interacts with multiple enzymatic components of DNA single-strand break repair (SSBR) including DNA kinase, DNA phosphatase, DNA polymerase, DNA deadenylase, and DNA ligase activities that collectively are capable of accelerating the repair of a broad range of DNA single-strand breaks (SSBs) (PMID: 18515191). XRCC1 is 633 amino acids in length which is comprised of an N-terminal domain (NTD) of ˜160 amino acids that interacts with Pol β, a central BRCT domain of ˜90 amino acids that interacts with PARP1 (PMID: 9584196). This antibody is not suitable for murine samples.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for XRCC1 antibody 87251-1-RR | Download protocol |
| WB protocol for XRCC1 antibody 87251-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |