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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XPR1 Recombinant monoclonal antibody (84902-1-RR)

    WB analysis using 84902-1-RR (same clone as 84902-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84902-1-RR (XPR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84902-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using XPR1 Recombinant monoclonal antibody (84902-1-RR)

    IP experiment of Jurkat using 84902-1-RR (same clone as 84902-1-PBS)

    IP result of anti-XPR1 (IP:84902-1-RR, 4ug; Detection:84902-1-RR 1:1000) with Jurkat cells lysate 1800 ug. This data was developed using the same antibody clone with 84902-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84902-1-RR

    Affinity and Kinetic Characterization of 84902-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84902-1-RR against Human XPR1 were performed. The affinity constant is below 1 pM.

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XPR1 Recombinant monoclonal antibody, PBS Only

XPR1 Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 84902-1-PBS
Clone No.241788A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

241788A7, Phosphate exporter SLC53A1, Protein SYG1 homolog, SLC53A1, Solute carrier family 53 member 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84902-1-PBS targets XPR1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen XPR1 fusion protein Ag5373 相同性解析による交差性が予測される生物種
Full Name xenotropic and polytropic retrovirus receptor
Calculated molecular weight 696 aa, 82 kDa
Observed molecular weight74 kDa
GenBank accession numberBC041142
Gene Symbol XPR1
Gene ID (NCBI) 9213
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBH6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

XPR1 is a receptor for xenotropic and polytropic murine leukemia retroviruses and a homolog of yeast Syg1 and plant Pi transporter PHO1 (PMID: 20633538). XPR1 has been identified as an atypical G-protein-coupled receptor. Xenotropic or polytropic retrovirus binding to XPR1 can disrupt the cAMP-mediated signaling function of Xpr1, leading to the apoptosis of infected cells (PMID: 22090134). A band of about 82-100 kDa is probably due to abnormal migration of the protein or post-translation modifications. In addition, a band of about 55-72 kDa may be isoforms and fragments.