Validation Data Gallery

Filter: AllWB

WB analysis of K-562 using 12980-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12980-1-AP (XPO7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 12980-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12980-1-AP (XPO7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12980-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12980-1-AP (XPO7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 12980-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12980-1-AP (XPO7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Featured Product
KD/KO Validated

XPO7 Polyclonal antibody

XPO7 Polyclonal Antibody for WB, ELISA

Cat No. 12980-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

Exp7, exportin 7, Exportin-7, KIAA0745, Ran binding protein 16

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

mouse brain tissue, mouse spleen tissue, K-562 cells, human brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 1 publications below

Product Information

12980-1-AP targets XPO7 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3603 Product name: Recombinant human XPO7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC030785 Sequence: MADHVQSLAQLENLCKQLYETTDTTTRLQAEKALVEFTNSPDCLSKCQLLLERGSSSYSQLLAATCLTKLVSRTNNPLPLEQRIDIRNYVLNYLATRPKLATFVTQALIQLYARITKLGWFDCQKDDYVFRNAITDVTRFLQDSVEYCIIGVTILSQLTNEINQADTTHPLTKHRKIASSFRDSSLFDIFTLSCNLLKQASGKNLNLNDESQHGLLMQLLKLTHNCLNFDFIGTSTDESSDDLCTVQIPTSWRSAFLDSSTLQLFFDLYHSIPPSFSPLVLSCLVQIASVRRSLFNNAER 相同性解析による交差性が予測される生物種
Full Name	exportin 7
Calculated molecular weight	1087 aa, 124 kDa
Observed molecular weight	100-110 kDa
GenBank accession number	BC030785
Gene Symbol	XPO7
Gene ID (NCBI)	23039
RRID	AB_2215493
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UIA9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for XPO7 antibody 12980-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Neuron Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes Authors - Norah Al-Azzam View Article
human	WB	Front Immunol A Mass Spectrometry-Based Profiling of Interactomes of Viral DDB1- and Cullin Ubiquitin Ligase-Binding Proteins Reveals NF-κB Inhibitory Activity of the HIV-2-Encoded Vpx. Authors - Christine D Landsberg View Article
human	WB,IF	Sci Rep TDP43 nuclear export and neurodegeneration in models of amyotrophic lateral sclerosis and frontotemporal dementia. Authors - Hilary C Archbold KD Validated View Article

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Validation Data Gallery

WB analysis of K-562 using 12980-1-AP

WB analysis using 12980-1-AP

WB analysis of human brain using 12980-1-AP

WB analysis of mouse spleen using 12980-1-AP

XPO7 Polyclonal antibody

XPO7 Polyclonal Antibody for WB, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes

A Mass Spectrometry-Based Profiling of Interactomes of Viral DDB1- and Cullin Ubiquitin Ligase-Binding Proteins Reveals NF-κB Inhibitory Activity of the HIV-2-Encoded Vpx.

TDP43 nuclear export and neurodegeneration in models of amyotrophic lateral sclerosis and frontotemporal dementia.

Able^™

Validation Data Gallery

WB analysis of K-562 using 12980-1-AP

WB analysis using 12980-1-AP

WB analysis of human brain using 12980-1-AP

WB analysis of mouse spleen using 12980-1-AP

XPO7 Polyclonal antibody

XPO7 Polyclonal Antibody for WB, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes

A Mass Spectrometry-Based Profiling of Interactomes of Viral DDB1- and Cullin Ubiquitin Ligase-Binding Proteins Reveals NF-κB Inhibitory Activity of the HIV-2-Encoded Vpx.

TDP43 nuclear export and neurodegeneration in models of amyotrophic lateral sclerosis and frontotemporal dementia.

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