Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IF | See 2 publications below |
Product Information
11408-1-AP targets XPO6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1898 Product name: Recombinant human XPO6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 539-888 aa of BC014071 Sequence: MPLDDTKLIIHQTLSVLEDIVENISGESTKSRQICYQSLQESVQVSLALFPAFIHQSDVTDEMLSFFLTLFRGLRVQMGVPFTEQIIQTFLNMFTREQLAESILHEGSTGCRVVEKFLKILQVVVQEPGQVFKPFLPSIIALCMEQVYPIIAERPSPDVKAELFELLFRTLHHNWRYFFKSTVLASVQRGIAEEQMENEPQFSAIMQAFGQSFLQPDIHLFKQNLFYLETLNTKQKLYHKKIFRTAMLFQFVNVLLQVLVHKSHDLLQEEIGIAIYNMASVDFDGFFAAFLPEFLTSCDGVDANQKSVLGRNFKMDRDLPSFTQNVHRLVNDLRYYRLCNDSLPPGTVKL 相同性解析による交差性が予測される生物種 |
| Full Name | exportin 6 |
| Calculated molecular weight | 1125 aa, 129 kDa |
| Observed molecular weight | 128 kDa |
| GenBank accession number | BC014071 |
| Gene Symbol | XPO6 |
| Gene ID (NCBI) | 23214 |
| RRID | AB_2241610 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96QU8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human XPO6 gene encodes a Ran-binding protein 20, exportin-6, belonging to the exportin family. Exportin-6 shuttles between the nucleus and the cytoplasm and mediates the nuclear export of actin and profilin-actin complexes in somatic cells. Interaction of exportin-6 and actin is achieved in a RanGTP-dependent manner, actin is a major cytoskeletal element which is normally kept cytoplasmic by exportin-6 driven nuclear export.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for XPO6 antibody 11408-1-AP | Download protocol |
| IP protocol for XPO6 antibody 11408-1-AP | Download protocol |
| WB protocol for XPO6 antibody 11408-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion. | ||
Proc Natl Acad Sci U S A Active maintenance of nuclear actin by importin 9 supports transcription. | ||
Cancer Res Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer. | ||
J Virol The XPO6 Exportin Mediates Herpes Simplex Virus 1 gM Nuclear Release Late in Infection.
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