Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33279-1-AP targets XPC in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38513 Product name: Recombinant human XPC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 685-867 aa of NM_004628.4 Sequence: HSRDTWLKKARVVRLGEVPYKMVKGFSNRARKARLAEPQLREENDLGLFGYWQTEEYQPPVAVDGKVPRNEFGNVYLFLPSMMPIGCVQLNLPNLHRVARKLDIDCVQAITGFDFHGGYSHPVTDGYIVCEEFKDVLLTAWENEQAVIERKEKEKKEKRALGNWKLLAKGLLIRERLKRRYGP 相同性解析による交差性が予測される生物種 |
| Full Name | xeroderma pigmentosum, complementation group C |
| Calculated molecular weight | 106kDa，940aa |
| Observed molecular weight | 106-120 kDa |
| GenBank accession number | NM_004628.4 |
| Gene Symbol | XPC |
| Gene ID (NCBI) | 7508 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q01831 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
XPC (DNA repair protein complementing XP-C cells) protein is a pivotal DNA damage recognition factor that plays an indispensable role in the global-genome nucleotide excision repair (GG-NER) pathway. As the primary initiator of GG-NER, it acts as a universal sensor for a wide array of helix-distorting DNA lesions, including those induced by ultraviolet (UV) radiation and chemical carcinogens. Upon binding to damaged DNA, the XPC complex orchestrates the recruitment of the entire repair machinery, serving as a critical guardian of genomic integrity. The calculated molecular weight of XPC is about 106 kDa, but due to post-translational modifications, about 120 kDa will be observed.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for XPC antibody 33279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |