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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using XKRX Polyclonal antibody (27049-1-AP)

    WB analysis of human placenta using 27049-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27049-1-AP (XKRX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using XKRX Polyclonal antibody (27049-1-AP)

    IHC staining of human placenta using 27049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27049-1-AP (XKRX Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using XKRX Polyclonal antibody (27049-1-AP)

    IHC staining of human placenta using 27049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27049-1-AP (XKRX Antibody) at dilution of 1:200 (under 40x lens).

XKRX Polyclonal antibody

XKRX Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27049-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Membrane protein XPLAC, X Kell blood group-related, X-linked, XK related protein 2, XKR2, XK-related protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27049-1-AP targets XKRX in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25266

Product name: Recombinant human XKRX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-210 aa of BC137010

Sequence: VHRDLAKDKPLSLFMHLILLGPVIRCLEAMIKYLTLWKKEEQEEPYVSLTRKKMLIDGEEVLIEWEVGHSIRTLAMHRNAYKRMSQIQAFLGSVPQLTYQLYVSLISAEV

相同性解析による交差性が予測される生物種
Full Name XK, Kell blood group complex subunit-related, X-linked
Calculated molecular weight 462 aa, 54 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC137010
Gene Symbol XKRX
Gene ID (NCBI) 402415
RRIDAB_2880732
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PP77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XKRX (XK-associated X-linked protein) is a component of the XK/Kell complex in the Kell blood group system, encoding a membrane protein with multiple transmembrane domains that is exposed on the cell surface and may function as a membrane transport protein. It is expressed predominantly in the placenta and syncytiotrophoblasts. It has 2 isoforms, with molecular weights of 52 kDa and 28 kDa respectively. Its function has not been fully clarified yet.

Protocols

Product Specific Protocols
WB protocol for XKRX antibody 27049-1-APDownload protocol
IHC protocol for XKRX antibody 27049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols