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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using XK Polyclonal antibody (12830-1-AP)

    WB analysis of mouse pancreas using 12830-1-AP (same clone as 12830-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12830-1-AP (XK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12830-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using XK Polyclonal antibody (12830-1-AP)

    WB analysis of MCF-7 using 12830-1-AP (same clone as 12830-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12830-1-AP (XK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using XK Polyclonal antibody (12830-1-AP)

    IHC staining of human pancreas cancer using 12830-1-AP (same clone as 12830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using XK Polyclonal antibody (12830-1-AP)

    IHC staining of human pancreas cancer using 12830-1-AP (same clone as 12830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using XK Polyclonal antibody (12830-1-AP)

    IHC staining of Human Colon cancer using 12830-1-AP (same clone as 12830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 12830-1-AP (XK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12830-1-PBS in a different storage buffer formulation.

XK Polyclonal antibody, PBS Only

XK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12830-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12830-1-PBS targets XK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3550

Product name: Recombinant human XK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 371-444 aa of BC036019

Sequence: QFFHPCKKLFSSSVSEGFQRWLRCFCWACRQQKPCEPIGKEDLQSSRDRDETPSSSKTSPEPGQFLNAEDLCSA

相同性解析による交差性が予測される生物種
Full Name X-linked Kx blood group (McLeod syndrome)
Calculated molecular weight 444 aa, 51 kDa
Observed molecular weight 45-51 kDa
GenBank accession numberBC036019
Gene Symbol XK
Gene ID (NCBI) 7504
RRIDAB_2877887
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51811
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

XK, also named as XKR1 and XRG1, is responsible for the Kx blood group system. XK forms a functional complex with the Kell glycoprotein. It represents a crucial factor for the maintenance of normal muscle structure and function.

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