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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XIRP2 Polyclonal antibody

XIRP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11896-1-AP

Synonyms

Beta xin, CMYA3, MYOMAXIN, Xeplin, XIRP2

Validation Data Gallery

Applications:
  • WB analysis of COLO 320 using 11896-1-AP

    WB analysis of COLO 320 using 11896-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 11896-1-AP

    WB analysis of human heart using 11896-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Caco-2 using 11896-1-AP

    WB analysis of Caco-2 using 11896-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 11896-1-AP

    IHC staining of human skeletal muscle using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 11896-1-AP

    IHC staining of human skeletal muscle using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 11896-1-AP

    IHC staining of human heart using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11896-1-AP

    IHC staining of human heart using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of mouse heart using 11896-1-AP

    IF Staining of mouse heart using 11896-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using XIRP2 antibody (11896-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells, Caco-2 cells, human heart tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11896-1-AP targets XIRP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XIRP2 fusion protein Ag2515 相同性解析による交差性が予測される生物種
Full Name xin actin-binding repeat containing 2
Calculated molecular weight 379 kDa
Observed molecular weight 260-300 kDa
GenBank accession numberBC022888
Gene symbol XIRP2
Gene ID (NCBI) 129446
RRIDAB_10951481
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for XIRP2 antibody 11896-1-APDownload protocol
IHC protocol for XIRP2 antibody 11896-1-APDownload protocol
IF protocol for XIRP2 antibody 11896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Elife

Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain

Authors - Elizabeth L Wagner
View Article
mouseWB,IF

Front Physiol

Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling.

Authors - Emily Farrell
View Article