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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XIAP Monoclonal antibody (66800-1-Ig)

    WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using XIAP Monoclonal antibody (66800-1-Ig)

    WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

    WB result of XIAP antibody (66800-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XIAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using XIAP Monoclonal antibody (66800-1-Ig)

    WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IF Staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using XIAP Monoclonal antibody (66800-1-Ig)

    IF Staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using XIAP Monoclonal antibody (66800-1-Ig)

    FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human XIAP (66800-1-Ig, Clone:2A1A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

XIAP Monoclonal antibody, PBS Only

XIAP Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66800-1-PBS
Clone No.2A1A12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

2A1A12, API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, EC:2.3.2.27

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66800-1-PBS targets XIAP in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag18088

Product name: Recombinant human XIAP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-497 aa of BC032729

Sequence: MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS

相同性解析による交差性が予測される生物種
Full Name X-linked inhibitor of apoptosis
Calculated molecular weight 60 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC032729
Gene Symbol XIAP
Gene ID (NCBI) 331
RRIDAB_2882143
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP98170
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.

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