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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XAGE2 Polyclonal antibody (33118-1-AP)

    WB analysis using 33118-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33118-1-AP (XAGE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using XAGE2 Polyclonal antibody (33118-1-AP)

    IF Staining of HeLa using 33118-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using XAGE2 antibody (33118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

XAGE2 Polyclonal antibody

XAGE2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 33118-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Cancer/testis antigen 12.2, CT12.2, G antigen family D member 3, GAGED3, X antigen family member 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCaco-2 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33118-1-AP targets XAGE2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35814

Product name: Recombinant human XAGE2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 17-111 aa of NM_130777

Sequence: LQPPELIGAMLEPTDEEPKEEKPPTKSRNPTPDQKREDDQGAAEIQVPDLEADLQELCQTKTGDGCEGGTDVKGKILPKAEHFKMPEAGEGKSQV

相同性解析による交差性が予測される生物種
Full Name X antigen family, member 2
Calculated molecular weight12kDa
Observed molecular weight20 kDa
GenBank accession numberNM_130777
Gene Symbol XAGE2
Gene ID (NCBI) 9502
RRIDAB_3742862
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96GT9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XAGE2 (member 2 of the X antigen family), also known as CT12.2, GAGED3, XAGE-2, and XAGE2B, is a cancer-testis (CT) antigen that belongs to the GAGE family. This protein is strongly expressed in normal testes and is abnormally expressed in various tumors, including Ewing's sarcoma, rhabdomyosarcoma, breast cancer, and germ cell tumors. The XAGE2 protein shares sequence similarity with other GAGE/PAGE proteins, and its expression pattern and sequence similarity make it a member of the CT antigen family.

Protocols

Product Specific Protocols
IF protocol for XAGE2 antibody 33118-1-APDownload protocol
WB protocol for XAGE2 antibody 33118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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