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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FRMD6/Willin Polyclonal antibody (21039-1-AP)

    WB analysis using 21039-1-AP (same clone as 21039-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21039-1-AP (Willin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FRMD6/Willin Polyclonal antibody (21039-1-AP)

    IHC staining of human liver cancer using 21039-1-AP (same clone as 21039-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FRMD6/Willin Polyclonal antibody (21039-1-AP)

    IHC staining of human liver cancer using 21039-1-AP (same clone as 21039-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using FRMD6/Willin Polyclonal antibody (21039-1-AP)

    IHC staining of human liver using 21039-1-AP (same clone as 21039-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using FRMD6/Willin Polyclonal antibody (21039-1-AP)

    IHC staining of human liver using 21039-1-AP (same clone as 21039-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using FRMD6/Willin Polyclonal antibody (21039-1-AP)

    FC experiment of A431 using 21039-1-AP (same clone as 21039-1-PBS)

    1X10^6 A431 cells were stained with .2ug Willin antibody (21039-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 21039-1-PBS in a different storage buffer formulation.

FRMD6/Willin Polyclonal antibody, PBS Only

FRMD6/Willin Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 21039-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

FRMD6, Willin, EX1, FERM domain containing 6, FERM domain-containing protein 6

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21039-1-PBS targets FRMD6/Willin in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15375

Product name: Recombinant human Willin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC029870

Sequence: LDMDQLEKRSRASGSSAGSMKHKRLSRHSTASHSSSHTSGIEADTKPRDTGPEDSYSSSAIHRKLKTCSSMTSHGSSHTSGVESGGKDRLEEDLQDDEIEMLVDDPRDLEQMNEESLEVSPDMCIYITEDMLMSRKLNGHSGLIVKEIGSSTSSSSETVVKLRGQSTDSLPQTICRKPKTSTDRHSLSLDDIRLYQKDFLRIAGLCQDTAQSYTFGCGHELDEEGLYCNSCLAQQCINIQDAFPVKRTSKYFSLDLTHDEVPEFVV

相同性解析による交差性が予測される生物種
Full Name FERM domain containing 6
Calculated molecular weight 622 aa, 72 kDa
Observed molecular weight72, 70, 30 kDa
GenBank accession numberBC029870
Gene Symbol FRMD6/Willin
Gene ID (NCBI) 122786
RRIDAB_2878797
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NE9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Willin, also known as FRMD6 or human Expanded (hEx), is a member of the FERM-domain family of cytoskeletal crosslinkers. It is a potent inhibitor of cell proliferation, transformation and modulator of drug sensitivity, suggesting it possesses tumor suppressor properties. Willin is widely expressed in normal tissues. Although willin is found in the cytoplasm and membrane, it localizes to the nuclei of almost all tumors.This antibody was raised against the C-terminal region of human FRMD6 protein and is expected to recognize the endogenous FRMD6.

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