×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using WWTR1/TAZ Recombinant monoclonal antibody (85245-5-RR)

    WB analysis using 85245-5-RR (same clone as 85245-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85245-5-RR (WWTR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85245-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using WWTR1/TAZ Recombinant monoclonal antibody (85245-5-RR)

    WB analysis of A549 using 85245-5-RR (same clone as 85245-5-PBS)

    WB result of WWTR1/TAZ antibody (85245-5-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWTR1/TAZ transfected A549 cells. This data was developed using the same antibody clone with 85245-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WWTR1/TAZ Recombinant monoclonal antibody (85245-5-RR)

    IF Staining of HeLa using 85245-5-RR (same clone as 85245-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WWTR1/TAZ antibody (85245-5-RR, Clone: 252185D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85245-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85245-5-RR

    Affinity and Kinetic Characterization of 85245-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85245-5-RR against Human WWTR1/TAZ were performed. The affinity constant is 2.97 nM.

WWTR1/TAZ Recombinant monoclonal antibody, PBS Only

WWTR1/TAZ Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85245-5-PBS
Clone No.252185D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

TAZ, WWTR1, Transcriptional coactivator with PDZ-binding motif, WW domain-containing transcription regulator protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85245-5-PBS targets WWTR1/TAZ in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6470

Product name: Recombinant Human WWTR1/TAZ protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-400 aa of NM_001168278.2

Sequence: MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL

相同性解析による交差性が予測される生物種
Full Name WW domain containing transcription regulator 1
Calculated molecular weight44 kDa
Observed molecular weight 50 kDa
GenBank accession numberNM_001168278.2
Gene Symbol WWTR1
Gene ID (NCBI) 25937
RRIDAB_3744121
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9GZV5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma (PMID: 22003437, PMID: 27764783). TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

||
New chat

Able™

正在加载，请稍候...