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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    WB analysis using 66500-1-Ig (same clone as 66500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    WB analysis of HepG2 using 66500-1-Ig (same clone as 66500-1-PBS)

    WB result of TAZ antibody (66500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HepG2 cells. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    WB analysis using 66500-1-Ig (same clone as 66500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    WB analysis of HSC-T6 using 66500-1-Ig (same clone as 66500-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    WB analysis of NIH/3T3 using 66500-1-Ig (same clone as 66500-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    IHC staining of human breast cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    IHC staining of human breast cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using WWTR1/TAZ Monoclonal antibody (66500-1-Ig)

    IF Staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TAZ antibody (66500-1-Ig, Clone: 2A12A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WWTR1/TAZ Monoclonal antibody, PBS Only

WWTR1/TAZ Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66500-1-PBS
Clone No.2A12A10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

TAZ, WWTR1, 2A12A10, Transcriptional coactivator with PDZ-binding motif, WW domain-containing transcription regulator protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66500-1-PBS targets WWTR1/TAZ in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag13330

Product name: Recombinant human WWTR1,TAZ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC014052

Sequence: MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL

相同性解析による交差性が予測される生物種
Full Name WW domain containing transcription regulator 1
Calculated molecular weight 44 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC014052
Gene Symbol WWTR1
Gene ID (NCBI) 25937
RRIDAB_2881864
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9GZV5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

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