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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis using 12609-1-AP (same clone as 12609-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of MCF-7 using 12609-1-AP (same clone as 12609-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of A431 using 12609-1-AP (same clone as 12609-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of rat kidney using 12609-1-AP (same clone as 12609-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of mouse kidney using 12609-1-AP (same clone as 12609-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using WT1 Polyclonal antibody (12609-1-AP)

    IP experiment of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    IP result of anti-WT1 (IP:12609-1-AP, 4ug; Detection:12609-1-AP 1:1000) with K-562 cells lysate 1360 ug. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using WT1 Polyclonal antibody (12609-1-AP)

    IF Staining of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using WT1 antibody (12609-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using WT1 Polyclonal antibody (12609-1-AP)

    FC experiment of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human WT1 (12609-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

WT1 Polyclonal antibody, PBS Only

WT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12609-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Wilms tumor 1, Wilms tumor protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12609-1-PBS targets WT1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WT1 fusion protein Ag3337 相同性解析による交差性が予測される生物種
Full Name Wilms tumor 1
Calculated molecular weight 449 aa, 49 kDa, 57 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC032861
Gene Symbol WT1
Gene ID (NCBI) 7490
RRIDAB_2216225
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19544
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.