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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WSTF Polyclonal antibody (26641-1-AP)

    WB analysis using 26641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26641-1-AP (WSTF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WSTF Polyclonal antibody (26641-1-AP)

    IF Staining of HeLa using 26641-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WSTF antibody (26641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

WSTF Polyclonal antibody

WSTF Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26641-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

BAZ1B, EC:2.7.10.2, hWALp2, Tyrosine protein kinase BAZ1B, Tyrosine-protein kinase BAZ1B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26641-1-AP targets WSTF in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24403

Product name: Recombinant human BAZ1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1144-1483 aa of BC136520

Sequence: KVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDMSAENARCKVCRKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPATARRNSRGRNYTEESASEDSEDDESDEEEEEEEEEEEEEDYEVAGLRLRPRKTIRGKHSVIPPAARSGRRPGKKPHSTRRSQPKAPPVDDAEVDELVLQTKRSSRRQSLELQKCEEILHKIVKYRFSWPFREPVTRDEAEDYYDVITHPMDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK

相同性解析による交差性が予測される生物種
Full Name bromodomain adjacent to zinc finger domain, 1B
Calculated molecular weight 1483 aa, 171 kDa
Observed molecular weight170 kDa
GenBank accession numberBC136520
Gene Symbol WSTF/BAZ1B
Gene ID (NCBI) 9031
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIG0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for WSTF antibody 26641-1-APDownload protocol
WB protocol for WSTF antibody 26641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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