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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using WSB2 Polyclonal antibody (12124-2-AP)

    WB analysis of PC-3 using 12124-2-AP (same clone as 12124-2-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using WSB2 Polyclonal antibody (12124-2-AP)

    WB analysis of human brain using 12124-2-AP (same clone as 12124-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using WSB2 Polyclonal antibody (12124-2-AP)

    WB analysis of mouse kidney using 12124-2-AP (same clone as 12124-2-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using WSB2 Polyclonal antibody (12124-2-AP)

    WB analysis of rat kidney using 12124-2-AP (same clone as 12124-2-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using WSB2 Polyclonal antibody (12124-2-AP)

    IP experiment of mouse kidney using 12124-2-AP (same clone as 12124-2-PBS)

    IP result of anti-WSB2 (IP:12124-2-AP, 3ug; Detection:12124-2-AP 1:800) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using WSB2 Polyclonal antibody (12124-2-AP)

    IF Staining of HEK-293 using 12124-2-AP (same clone as 12124-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using WSB2 antibody (12124-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WSB2 Polyclonal antibody, PBS Only

WSB2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 12124-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12124-2-PBS targets WSB2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2768

Product name: Recombinant human WSB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-404 aa of BC015887

Sequence: MEAGEEPLLLAELKPGRPHQFDWKSSCETWSVAFSPDGSWFAWSQGHCIVKLIPWPLEEQFIPKGFEAKSRSSKNETKGRGSPKEKTLDCGQIVWGLAFSPWPSPPSRKLWARHHPQVPDVSCLVLATGLNDGQIKIWEVQTGLLLLNLSGHQDVVRDLSFTPSGSLILVSASRDKTLRIWDLNKHGKQIQVLSGHLQWVYCCSISPDCSMLCSAAGEKSVFLWSMRSYTLIRKLEGHQSSVVSCDFSPDSALLVTASYDTNVIMWDPYTGERLRSLHHTQVDPAMDDSDVHISSLRSVCFSPEGLYLATVADDRLLRIWALELKTPIAFAPMTNGLCCTFFPHGGVIATGTRDGHVQFWTAPRVLSSLKHLCRKALRSFLTTYQVLALPIPKKMKEFLTYRTF

相同性解析による交差性が予測される生物種
Full Name WD repeat and SOCS box-containing 2
Calculated molecular weight 404 aa, 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC015887
Gene Symbol WSB2
Gene ID (NCBI) 55884
RRIDAB_2216206
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYS7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WSB2, also named SBA2, encodes WD repeat and SOCS box-containing protein 2. WSB2 may be a substrate-recognition component of an E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. WSB2 is a novel protein supposed to be involved in male- and female-specific gonadal development, and also a regulator of IL-21 receptor expression and IL21-induced signal transduction. WSB2 might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological functions need further studies.

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