Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Raji cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00057 targets WRAP53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat containing, antisense to TP53 |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 72-75 kDa |
| GenBank accession number | BC002336 |
| Gene Symbol | WRAP53 |
| Gene ID (NCBI) | 55135 |
| RRID | AB_3750108 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | Q9BUR4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WRAP53 (WD40-encoding RNA Antisense to p53), also known as TCAB1 and WDR79, is a multifunctional protein encoded by a gene that produces both a coding mRNA and a regulatory antisense RNA targeting the tumor suppressor p53. Initially identified for its role in maintaining genomic stability by regulating p53 expression, WRAP53 is crucial for telomerase assembly and localization to Cajal bodies, impacting telomere maintenance. It also participates in DNA damage repair and cellular stress responses. The calculated molecular weight of WRAP53 is about 59 kDa, but the actual observed molecular weight is about 72-75 kDa, which may be due to modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for WRAP53 antibody RMX00057 | Download protocol |
| IHC protocol for WRAP53 antibody RMX00057 | Download protocol |
| WB protocol for WRAP53 antibody RMX00057 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |