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Validation Data Gallery

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  • Western Blot (WB) analysis of SGC-7901 cells using WNT6 Polyclonal antibody (24201-1-AP)

    WB analysis of SGC-7901 using 24201-1-AP (same clone as 24201-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24201-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WNT6 Polyclonal antibody (24201-1-AP)

    IHC staining of mouse brain using 24201-1-AP (same clone as 24201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24201-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WNT6 Polyclonal antibody (24201-1-AP)

    IHC staining of mouse brain using 24201-1-AP (same clone as 24201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24201-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using WNT6 Polyclonal antibody (24201-1-AP)

    IHC staining of human small intestine using 24201-1-AP (same clone as 24201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24201-1-AP (WNT6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24201-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using WNT6 Polyclonal antibody (24201-1-AP)

    IHC staining of human small intestine using 24201-1-AP (same clone as 24201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24201-1-AP (WNT6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24201-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WNT6 Polyclonal antibody, PBS Only

WNT6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24201-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24201-1-PBS targets WNT6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20889

Product name: Recombinant human WNT6 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 36-365 aa of BC004329

Sequence: DPTSICRKARRLAGRQAELCQAEPEVVAELARGARLGVRECQFQFRFRRWNCSSHSKAFGRILQQDIRETAFVFAITAAGASHAVTQACSMGELLQCGCQAPRGRAPPRPSGLPGTPGPPGPAGSPEGSAAWEWGGCGDDVDFGDEKSRLFMDARHKRGRGDIRALVQLHNNEAGRLAVRSHTRTECKCHGLSGSCALRTCWQKLPPFREVGARLLERFHGASRVMGTNDGKALLPAVRTLKPPGRADLLYAADSPDFCAPNRRTGSPGTRGRACNSSAPDLSGCDLLCCGRGHRQESVQLEENCLCRFHWCCVVQCHRCRVRKELSLCL

相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 6
Calculated molecular weight 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC004329
Gene Symbol WNT6
Gene ID (NCBI) 7475
RRIDAB_2879454
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6F9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WNT6 belongs to the Wnt family. It is ligand for members of the frizzled family of seven transmembrane receptors. WNT6 is likely to signal over only few cell diameters. WNT6 may be together with CAV1 to promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway. And WNT6 is mainly expressed in brain, endometrium epithelium, gastric cancer cell lines and gastric cancer tissues (PMID: 22370641).

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