Validation Data Gallery

Filter: AllWBIHC

WB analysis using 55184-1-AP (same clone as 55184-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
WB analysis using 55184-1-AP (same clone as 55184-1-PBS)
HUVEC cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 55184-1-AP (same clone as 55184-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 55184-1-AP (same clone as 55184-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

WNT5A/B Polyclonal antibody, PBS Only

WNT5A/B Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55184-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

WNT5A, hWNT5A, Protein Wnt 5a, Protein Wnt-5a, WNT5A B

Formulation: PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55184-1-PBS targets WNT5A/B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	wingless-type MMTV integration site family, member 5A
Calculated molecular weight	42 kDa
Observed molecular weight	38-42 kDa
GenBank accession number	NM_003392
Gene Symbol	WNT5A
Gene ID (NCBI)	7474
RRID	AB_2881285
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P41221
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides. This antibody can recognize WNT5A and WNT5B.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 55184-1-AP (same clone as 55184-1-PBS)

WB analysis using 55184-1-AP (same clone as 55184-1-PBS)

WB analysis of PC-3 using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of mouse heart using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of human thyroid cancer using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of human thyroid cancer using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of human tonsillitis using 55184-1-AP (same clone as 55184-1-PBS)

IHC staining of human tonsillitis using 55184-1-AP (same clone as 55184-1-PBS)

WNT5A/B Polyclonal antibody, PBS Only

WNT5A/B Polyclonal Antibody for WB, IHC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

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