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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WNT5A/B Polyclonal antibody (29793-1-AP)

    WB analysis using 29793-1-AP (same clone as 29793-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A/B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29793-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WNT5A/B Polyclonal antibody (29793-1-AP)

    WB analysis using 29793-1-AP (same clone as 29793-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29793-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WNT5A/B Polyclonal antibody (29793-1-AP)

    IF Staining of HeLa using 29793-1-AP (same clone as 29793-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT5A antibody (29793-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29793-1-PBS in a different storage buffer formulation.

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WNT5A/B Polyclonal antibody, PBS Only

WNT5A/B Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 29793-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

WNT5A, hWNT5A, Protein Wnt 5a, Protein Wnt-5a, WNT5A B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29793-1-PBS targets WNT5A/B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT5A/B fusion protein Ag31507 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 5A
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC064694
Gene Symbol WNT5A
Gene ID (NCBI) 7474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41221
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides.