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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using WNT4 Polyclonal antibody (14371-1-AP)

    IHC staining of human kidney using 14371-1-AP (same clone as 14371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using WNT4 Polyclonal antibody (14371-1-AP)

    IHC staining of human kidney using 14371-1-AP (same clone as 14371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using WNT4 Polyclonal antibody (14371-1-AP)

    IHC staining of human kidney using 14371-1-AP (same clone as 14371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using WNT4 Polyclonal antibody (14371-1-AP)

    IHC staining of human hepatocirrhosis using 14371-1-AP (same clone as 14371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using WNT4 Polyclonal antibody (14371-1-AP)

    IHC staining of human hepatocirrhosis using 14371-1-AP (same clone as 14371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using WNT4 Polyclonal antibody (14371-1-AP)

    IHC staining of mouse kidney using 14371-1-AP (same clone as 14371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using WNT4 Polyclonal antibody (14371-1-AP)

    IHC staining of mouse kidney using 14371-1-AP (same clone as 14371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WNT4 Polyclonal antibody (14371-1-AP)

    IF Staining of HeLa using 14371-1-AP (same clone as 14371-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using WNT4 Polyclonal antibody (14371-1-AP)

    IF Staining of MCF-7 using 14371-1-AP (same clone as 14371-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14371-1-PBS in a different storage buffer formulation.

WNT4 Polyclonal antibody, PBS Only

WNT4 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 14371-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

WNT 4, UNQ426/PRO864, SERKAL, Protein Wnt-4, Protein Wnt 4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14371-1-PBS targets WNT4 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5741

Product name: Recombinant human WNT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC057781

Sequence: MSPRSCLRSLRLLVFAVFSAAASNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDMRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCSCKFHWCCFVKCRQCQRLVELHTCR

相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 4
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC057781
Gene Symbol WNT4
Gene ID (NCBI) 54361
RRIDAB_2215428
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP56705
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WNT4 belongs to the Wnt family. WNT4 is critical for embryonic organogenesis, but later plays a unique role among Wnt proteins in the development and maintenance of müllerian and reproductive tissues (PMID: 33963381). The mesenchymal stem cells can improve cardiac function after cardiac injury by regulating endothelial cells via the Wnt4/β-catenin signaling pathway (PMID: 20551912). WNT4 may be modified by glycosylation, leading to an increase in molecular weight.

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