Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig brain tissue, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
67452-1-Ig targets WNT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27957 Product name: Recombinant human WNT3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 130-306 aa of BC114219 Sequence: SCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHG 相同性解析による交差性が予測される生物種 |
| Full Name | wingless-type MMTV integration site family, member 3 |
| Calculated molecular weight | 355 aa, 40 kDa |
| GenBank accession number | BC114219 |
| Gene Symbol | WNT3 |
| Gene ID (NCBI) | 7473 |
| RRID | AB_2882686 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P56703 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WNT3 antibody 67452-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int miR-454 suppresses the proliferation and invasion of ovarian cancer by targeting E2F6. | ||
Chin J Integr Med Babao Dan () Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway. | ||
Kidney Int Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis | ||
World J Gastrointest Oncol N-glycosylation of Wnt3 regulates the progression of hepatocellular carcinoma by affecting Wnt/β-catenin signal pathway |
