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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using WNK4 Recombinant antibody (85886-4-RR)

    WB analysis of HepG2 using 85886-4-RR (same clone as 85886-4-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85886-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WNK4 Recombinant antibody (85886-4-RR)

    WB analysis using 85886-4-RR (same clone as 85886-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85886-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85886-4-RR

    Affinity and Kinetic Characterization of 85886-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85886-4-RR against Human WNK4 were performed. The affinity constant is 0.926 nM.

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WNK4 Recombinant antibody, PBS Only

WNK4 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85886-4-PBS
Clone No.250156A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:2.7.11.1, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Serine/threonine-protein kinase WNK4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85886-4-PBS targets WNK4 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WNK4 fusion protein Ag17773 相同性解析による交差性が予測される生物種
Full Name WNK lysine deficient protein kinase 4
Calculated molecular weight 1243 aa, 135 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC136664
Gene Symbol WNK4
Gene ID (NCBI) 65266
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96J92
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

WNK4 (With No Lysine Kinase 4) is a member of the WNK family of serine/threonine protein kinases. It plays a crucial role in regulating electrolyte balance and blood pressure by modulating the activity of ion transporters in the kidney. WNK4 is primarily involved in the regulation of sodium and potassium homeostasis. It achieves this by phosphorylating and activating the kinases SPAK and OSR1, which in turn regulate the activity of the sodium-chloride cotransporter (NCC) in the distal convoluted tubule of the kidney. Additionally, by promoting sodium reabsorption and potassium secretion, WNK4 contributes to the regulation of blood pressure. Increased activity of WNK4 can lead to hypertension. (PMID: 24655003; 33491560)