Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32231-1-AP targets WNK3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37713 Product name: Recombinant mouse WNK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 500-650 aa of NM_001002838 Sequence: AGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQST 相同性解析による交差性が予測される生物種 |
| Full Name | WNK lysine deficient protein kinase 3 |
| Calculated molecular weight | 1800aa, 198 kDa |
| Observed molecular weight | 230 kDa |
| GenBank accession number | NM_001002838 |
| Gene Symbol | WNK3 |
| Gene ID (NCBI) | 65267 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9BYP7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WNK3, is a member of the WNK (With No Lysine [K]) kinase family. It is a serine/threonine kinase that plays important roles in various physiological processes. WNK3 is expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain-specific. Isoform 3 is kidney-specific. Endogenous WNK3 protein is an active protein kinase when immunoprecipitated from cells and its overexpression increases the survival of HeLa cells by delaying the onset of apoptosis (PMID:16501604).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WNK3 antibody 32231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |