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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WNK2 Polyclonal antibody

WNK2 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22297-1-AP

Synonyms

Antigen NY CO 43, KIAA1760, NY CO 43, P/OKcl.13, PRKWNK2, SDCCAG43, WNK2

Validation Data Gallery

Applications:
  • IHC staining of human heart using 22297-1-AP

    IHC staining of human heart using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 22297-1-AP

    IHC staining of human heart using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 22297-1-AP

    IHC staining of human lung cancer using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 22297-1-AP

    IHC staining of human lung cancer using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 22297-1-AP

    IF Staining of HepG2 using 22297-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22297-1-AP (WNK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

22297-1-AP targets WNK2 in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name WNK lysine deficient protein kinase 2
Calculated molecular weight 243 kDa
GenBank accession numberNM_006648
Gene symbol WNK2
Gene ID (NCBI) 65268
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for WNK2 antibody 22297-1-APDownload protocol
IF protocol for WNK2 antibody 22297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols