Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32378-1-AP targets WIZ in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37337 Product name: Recombinant human WIZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 530-745 aa of BC136641 Sequence: YLGSVAAKRPLQEDRLLPAEVKAKTYIQTELPFKAKTLHEKTSHSSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIKHRPQKVGAYRSYIQGGRPFTKKFRSAGHGRDSDKRPSLGLAPGGLAVVGRSAGGEPGPEAGRAADGGERPLAASPPGTVKAEEHQRQNINKFERRQARPPDASAARGGEDTNDLQQKLEEV 相同性解析による交差性が予測される生物種 |
| Full Name | widely interspaced zinc finger motifs |
| GenBank accession number | BC136641 |
| Gene Symbol | WIZ |
| Gene ID (NCBI) | 58525 |
| RRID | AB_3742588 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O95785 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The WIZ protein, whose full name is "Widely-interspaced zinc finger-containing protein," belongs to the Krueppel-type C2H2 zinc finger protein family. Its core function is to act as a chromatin-associated transcription factor that operates within the nucleus, where it links the histone methyltransferases EHMT1 and EHMT2 with the CTBP transcriptional corepressor complex, thereby participating in the regulation of gene transcription activity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for WIZ antibody 32378-1-AP | Download protocol |
| IHC protocol for WIZ antibody 32378-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |