Caco-2 cells were subjected to SDS PAGE followed by western blot with 21259-1-AP (WISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21259-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21259-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21259-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21259-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21259-1-PBS in a different storage buffer formulation.