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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WIPF1 Polyclonal antibody

WIPF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22047-1-AP

Synonyms

Protein PRPL 2, PRPL 2, WASP interacting protein, WASPIP, WIP, WIPF1

Validation Data Gallery

Applications:
  • WB analysis using 22047-1-AP

    WB analysis using 22047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22047-1-AP (WIPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 22047-1-AP

    IHC staining of human stomach cancer using 22047-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 22047-1-AP

    IHC staining of human stomach cancer using 22047-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

22047-1-AP targets WIPF1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WIPF1 fusion protein Ag16975 相同性解析による交差性が予測される生物種
Full Name WAS/WASL interacting protein family, member 1
Calculated molecular weight403aa,40 kDa; 503aa,51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC110288
Gene symbol WIPF1
Gene ID (NCBI) 7456
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WASP interacting protein family member 1(WIPF1), also known as WIP, is a ubiquitously expressed proline-rich multidomain protein and plays a role in the reorganization of the actin cytoskeleton. Mutations in the WIPF1 binding site of the Wiskott-Aldrich syndrome protein or in WIPF1 itself cause Wiskott-Aldrich syndrome, which predisposes people to cancer, such as leukemia and lymphoma(PMID: 27863429).

Protocols

Product Specific Protocols
WB protocol for WIPF1 antibody 22047-1-APDownload protocol
IHC protocol for WIPF1 antibody 22047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols