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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WIBG Recombinant antibody (86064-1-RR)

    WB analysis using 86064-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using WIBG Recombinant antibody (86064-1-RR)

    WB analysis using 86064-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86064-1-RR (WIBG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using WIBG Recombinant antibody (86064-1-RR)

    IHC staining of mouse colon using 86064-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86064-1-RR (WIBG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using WIBG Recombinant antibody (86064-1-RR)

    IF Staining of MCF-7 using 86064-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using WIBG antibody (86064-1-RR, Clone: 242800G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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WIBG Recombinant antibody

WIBG Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86064-1-RR
Clone No.242800G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Partner of Y14 and mago, Protein wibg homolog, PYM, PYM homolog 1 exon junction complex-associated factor, PYM1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, SH-SY5Y cells, HepG2 cells, U-251 cells, HL-60 cells, mouse thymus tissue, mouse spleen tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86064-1-RR targets WIBG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WIBG fusion protein Ag6276 相同性解析による交差性が予測される生物種
Full Name within bgcn homolog (Drosophila)
Calculated molecular weight 23 kDa
Observed molecular weight23-27 kDa
GenBank accession numberBC006135
Gene Symbol WIBG
Gene ID (NCBI) 84305
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BRP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).

Protocols

Product Specific Protocols
WB protocol for WIBG antibody 86064-1-RRDownload protocol
IHC protocol for WIBG antibody 86064-1-RRDownload protocol
IF protocol for WIBG antibody 86064-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols